Location: B204 Microbiology Building

Zeiss LSM 510 META laser scanning confocal microscope equipped with 4 lasers This microscope has a META multi-spectral analyzer and a dual laser scanning system. Wavelengths available are 405 nm, 458 nm, 488 nm, 514 nm, 543 nm and 633nm. This instrument has a stage incubator for live cell imaging and Zeiss imaging software to capture and process images.

The automated and reproducible image capture is performed by three photomultipliers (2 standard PMT + 1 META) plus separate PMT for visible light which is recorded simultaneously; and simultaneous interlaced dual laser scanning (“multi-tracking” not available in other confocal systems) which permits simultaneous monitoring of 3-label specimens. However, up to 8 different channels can be imaged if done sequentially. The LSM 510 scans at 5.2 Frames per second (FPS); 512 x 512, and 77 FPS at 512 x 32 pixels. Line scans at 2800hz. The maximum image size is of 2048×2048 RGB= 12MByte.

For Training and use of this equipment contact: Mercedes Gonzalez-Juarrero (970-491-7306; Mercedes.gonzalez-juarrero@colostate.edu)

Cost

         Training: $250 including 5 h of use.

         Hourly: $20/h

         Subscription: $400/ first month, $300 second month, then $210/month.