Location: Room 221A MRB. Relocation planned for fall 2024 to room 236 MRB.

For high magnification with good depth of field and fluorescence capability an Olympus SZX16 stereo microscope is available. This microscope has a two-position revolving nosepiece equipped with a 0.8X PlanApo objective with an 8.1 cm working distance. The second objective is a 2X PlanApo objective (2-3 cm working distance) with zoom capabilities to over 300x allowing visualization of single fluorescence cells within a tissue slice.  The microscope has a base transillumination system with a rotator holding 4 cartridges for different types of illumination: a low oblique illumination cartridge ideal for many dissection operations, a bright field standard contrast cartridge, a dark field cartridge and a polarization cartridge.  Several of these cartridges are tilt-adjustable to obtain optimal performance through a front-mounted knob on the base.  The microscope has a 50/50 split between camera and eyepieces allowing video display/recording of dissection procedures. Fluorescence is from an LED direct coupled system with two filter sets installed, one for GFP (ex at 470/40 and em at 525/50) and one for TRITC (ex at 545/30 and em at 620/60) and an unmounted set for ex at 560/40 and em at 630/75).  Other filter sets (9 available) can be purchased for excitation as low as 380 nm.  The microscope is equipped with an EP50 5 MP color CMOS camera for wireless connections to up to 5 devices within wireless range. The EPviewer software can be downloaded for either a Mac or pc from the following locations:

mac: https://www.olympus-lifescience.com/en/camera/color/ep50/

pc: https://www.olympus-lifescience.com/en/support/downloads/#idlOpen=%23detail847252369

The microscope is currently available in a laminar flow hood in room 221A MRB for sterile dissection and cell/tissue labeling/imaging studies. In the fall of 2024, it will be available for use in in a room 236 MRB at which time it will be added to a scheduling calendar for trained user access.

For training and use of this equipment contact: Jim Bamburg (970-988-9120; james.bamburg@colostate.edu)

Cost:

         Training: $25

         Hourly: TBD after relocation