Location: Room 236 MRB.

This microscope has excellent depth of field and fluorescence capability as well as several transmitted light modes and a wireless video camera for recording on a personal laptop computer.  A two-position revolving nosepiece is equipped with a 0.8X PlanApo objective with an 8.1 cm working distance and a 2X PlanApo objective (2-3 cm working distance) with zoom capabilities to 230x allowing visualization of single fluorescent cells within a tissue slice or cell culture.  The base transillumination system has a rotator holding 4 cartridges: low oblique illumination ideal for many dissection operations; bright field standard contrast cartridge; dark field cartridge; polarization cartridge.  Some of these cartridges are tilt-adjustable to obtain optimal performance through a front-mounted knob on the base.  The microscope has a 50/50 split between camera and eyepieces allowing video display/recording of dissection procedures. Overhead white light illumination with fiber optics or side-illumination of slides for dark-field or light scattering, are also available. Fluorescence is from an LED direct coupled system with two filter sets installed, one for GFP (470/40 ex and 525/50 em) and one for TRITC (545/30 ex and 620/60 em) and an unmounted set for 560/40 ex and 630/75 em).  Other filter sets (9 available) can be purchased for excitation as low as 380 nm.  The microscope is equipped with an EP50 5 MP color CMOS camera for wireless connections to up to 5 devices within wireless range. The EPviewer software can be downloaded for either a Mac or PC from the following locations and should be done in advance for anyone wanting to get trained on this microscope.:

mac: https://www.olympus-lifescience.com/en/camera/color/ep50/

pc: https://www.olympus-lifescience.com/en/support/downloads/#idlOpen=%23detail847252369

The microscope is in a laminar flow hood for sterile dissection and cell/tissue labeling/imaging studies but does not protect the user from exposure to infected or toxic samples.  

For training and use of this equipment contact: Soham Chanda(soham.chanda@colostate.edu)

Cost:

         Training: $25

         Hourly: $1/hr or $5/day