Location: Room E317 A/Z

This system is used for preparing tissue slices and for their culture under conditions in which they can be removed and repetitively imaged over days to months. A special MOAI facility in A/Z East contains a large roller incubator for the long- term culture of slices in specially designed adapters to hold roller tubes. Slices are mounted on coverslips treated to adhere tissue and then affixed with Secure Seal to the flat side of a roller tube over a drilled hole. Medium is then added and the tube rotated in the incubator. A specially designed stage adapter allows intermittent imaging of the slices for fluorescence or dye label. The same groups of labeled neurons in a rodent hippocampal slice have been followed for many days but slices maintain their viability for weeks to months. See: Fixman et al., J Vis Exp. 2017 Dec 28;(130):56436. doi: 10.3791/56436.  Contact Rick McCosh in Biomedical Sciences. (Richard.McCosh@colostate.edu)