Plasma Cleaner: A Plasma Etch PE-25 plasma cleaner for preparing coverslips is located in room 245A of the MRB building (Markus lab). There is currently no charge for its use, and it may be used by anyone. Its application is mainly for preparing dust-free and fluorescence-free coverslips to be used for single molecule imaging.  For training and use contact: Dr. Steven Markus (steven.markus@colostate.edu)

Microelectrode Puller: A Campden Instruments moving-coil microelectrode puller (Model 753) is available in Room 224 of the MRB building. It can be used for making cell injection pipettes. For training and use contact: James.Bamburg@colostate.edu

Special Stage Adapters for Tissue Slice Culture Imaging with controlled CO2/temperature: The stage adapter holds flat sides stubes modified to hold tissue slices that attach to a coverslip sealed to the outside of the flat side of the tube for use on the Keyence Microscope. See: Fixman et al., J Vis Exp. 2017 Dec 28;(130):56436. doi: 10.3791/56436.  Design of devices for long term culture of tissue slices in a roller incubator are included and some are available from the MOAI Director upon request. Modified tubes can be used to change nitrogen/oxygen and CO₂ levels to emulate ischemic injury while observing the tissue.  A laminar-flow hood with a stereomicroscope for dissection, a McIlwain Tissue Chopper for preparing slices, and a roller tune incubator will be available in the fall of 2024 in room 236 MRB.  Fee for training and use: $250. Contact: James.bamburg@colostate.edu

Physitemp TS4 Piezo Stage and Controller: This stage adapter holds only one 35 mm culture dish (without strengthening ring) with drilled out hole and mounted coverslip. The stage heats and cools rapidly and a thermistor for measuring the medium temperature is available. Max temperature is 60^o C and minimum is -20^o C. The stage can be used to observe cells while cooling or heating. Maximum objective power is 40x and no oil immersion objectives can be used with the stage because of distance limitations to access the coverslip. It can be used on either the Nikon epifluorescence microscope of the Keyence microscope (requires special handling for installation only by James Bamburg).

Fiber-Lite Illuminator for Side Illumination of Glass Slides: This side illumination system was designed to enhance the detection of silver grains in emulsions overlaid on cells or tissues that have been radioisotope labeled. However, it can also be useful for illumination of samples in which dark field imaging works well or combined with polarization filter for reflective light on the Olympus Stereozoom Microscope. Contact: James.Bamburg@colostate.edu

Micromanipulators: Two different styles of micromanipulators are available, one with X,Y Z control from three different dials and the other with a joystick control. May be signed out from MRB 224. Contact alisa.shaw@colostate.edu

Grass Stimulator: This stimulator can be used for whole culture electrical stimulation at different frequencies and intensities for studying neuronal network excitation. May be signed out from MRB 224. Contact alisa.shaw@colostate.edu

Location: The following are located in Molecular and Radiological Biosciences
(MRB)Plasma Cleaner: A Plasma Etch PE-25
plasma cleaner for preparing coverslips is located in room 245A of the MRB
building (Markus lab). There is currently no charge for its use, and it may be
used by anyone. Its application is mainly for preparing dust-free and
fluorescence-free coverslips to be used for single molecule imaging.  For training and use contact: Dr. Steven
Markus (steven.markus@colostate.edu)Microelectrode Puller: A Campden
Instruments moving-coil microelectrode puller (Model 753) is available in Room
224 of the MRB building. It can be used for making cell injection pipettes. For
training and use contact: James.Bamburg@colostate.eduSpecial Stage Adapters for Tissue
Slice Culture Imaging with controlled CO2/temperature: The stage adapter
holds flat sides stubes modified to hold tissue slices that attach to a
coverslip sealed to the outside of the flat side of the tube for use on the
Keyence Microscope. See: Fixman et al., J Vis Exp. 2017 Dec 28;(130):56436.
doi: 10.3791/56436.  Design of devices
for long term culture of tissue slices in a roller incubator are included and
some are available from the MOAI Director upon request. Modified tubes can be
used to change nitrogen/oxygen and CO₂ levels to emulate ischemic
injury while observing the tissue.  A
laminar-flow hood with a stereomicroscope for dissection, a McIlwain Tissue
Chopper for preparing slices, and a roller tune incubator will be available in
the fall of 2024 in room 236 MRB.  Fee
for training and use: $250. Contact: James.bamburg@colostate.eduPhysitemp TS4 Piezo Stage and Controller: This stage adapter
holds only one 35 mm culture dish (without strengthening ring) with drilled out
hole and mounted coverslip. The stage heats and cools rapidly and a thermistor
for measuring the medium temperature is available. Max temperature is 60^o
C and minimum is -20^o C. The stage can be used to observe cells
while cooling or heating. Maximum objective power is 40x and no oil immersion
objectives can be used with the stage because of distance limitations to access
the coverslip. It can be used on either the Nikon epifluorescence microscope of
the Keyence microscope (requires special handling for installation only by
James Bamburg).Fiber-Lite Illuminator for Side Illumination of Glass Slides: This side
illumination system was designed to enhance the detection of silver grains in
emulsions overlaid on cells or tissues that have been radioisotope labeled. However,
it can also be useful for illumination of samples in which dark field imaging
works well or combined with polarization filter for reflective light on the
Olympus Stereozoom Microscope. Contact: James.Bamburg@colostate.eduMicromanipulators: Two different styles of micromanipulators are
available, one with X,Y Z control from three different dials and the other with
a joystick control. May be signed out from MRB 224. Contact
alisa.shaw@colostate.eduGrass Stimulator: This stimulator can be used for whole culture
electrical stimulation at different frequencies and intensities for studying
neuronal network excitation. May be signed out from MRB 224. Contact
alisa.shaw@colostate.edu