Location: Room 224 Molecular and Radiological Biosciences (MRB) Building

The Olympus IX83 Inverted Spinning Disk Confocal (SDC) Microscope includes a Zero Drift Compensation (ZDC) autofocusing unit. The microscope has a stage temperature and CO2 control for live cell imaging on 35 mm glass bottom dishes and glass bottom microtiter plates. The spinning disc unit is a Yokagawa CSU22 system with quad dichroic filters and the microscope has an additional emission filter wheel to eliminate spectral crossover. Laser lines for excitation are available at 405 nm, 488 nm, 561 nm and 647 nm with rapid (microsecond) switcher and a phasor holographic photobleaching/ photoactivation/photoconversion system for intracellular molecular dynamic measurements. The X,Y, piezo Z stage allows rapid image stack acquisition across many predetermined fields (4 D imaging), The Phasor can also be used for ion uncaging and other applications. System has differential interference contrast (DIC) optics with 10, 20, 40x air, and 60 and 100X oil objectives, built in correction for spherical aberration for all objectives, and a wide field Xenon light source.  An Andor iXon EMCCD camera for confocal imaging and a Photometrics HQ camera for wide field imaging are both integrated for image capture using Slidebook software.  Laser selection and power are software controlled.

For training and use of this equipment contact: Alisa Shaw, 970-443-5370 (alisa.shaw@colostate.edu)

Cost:

         Training: $250 includes 5 h of use.

         Hourly: $20

         Subscription: $400/ first month, $300 second month, then $210/month.