COMING SOON (Jan. 2026)

Location: Room 224 MRB. Installation expected before 12/31/25.

This specially configured inverted super-resolution microscope is designed for state-of-the-art fluorescence imaging of intracellular molecular dynamics for use with a broad range of biological model systems including yeast, animal cells, organoids, and nematodes. It is capable of rapid 3D spinning disk confocal imaging with super-resolution (Yokagawa SoRA) using a 50 μm pinhole disk combined with a dual microlens disk for 3x brighter imaging than standard SDC systems, meaning less phototoxicity or, alternatively, faster image acquisition. Additionally, it features rapid control of multiple devices using a fiber switcher for millisecond path switching, a laser stack with a modular laser combiner (405, 488, 561 and 638 nm lasers), a phasor for rapid 3D photobleaching, photoactivation, or photoconversion, and a Vector3 total internal reflection fluorescence (TIRF) illuminator (laser-based point scanner for both TIRF and photomanipulation using a 100X 1.46NS TIRF objective) that can be combined with spinning disk confocal imaging using a rapid-acting beam splitter. The unique ability of this microscope to rapidly switch between confocal and TIRF/HILO imaging modes provides the ability to track single molecules while simultaneously co-imaging nearby structures with confocality. The system also includes dual cameras for simultaneously maximizing imaging signal-to-noise (EMCCD) and speed/field-of-view (CMOS). Also featured full temperature and atmospheric (CO₂) control and stage holders for microtiter plates, dishes and slides.

Training and costs will be updated after installation.